Primer
Part:BBa_K3658003:Design
Designed by: Marko Susic Group: iGEM20_NYU_Abu_Dhabi (2020-10-24)
Batrachochytrium dendrobatidis (Bd) ITS region RPA forward primer
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The primers generated were annoated in Benchling and optimal primers were selected based on location, GC content, fitness with PCR amplicon, and quality of available gRNAs within the amplified region.
Source
We found the Bd ITS region sequence in NCBI and used primer-BLAST and PrimedRPA software to design the primer.